Kwang-sun Kim 사진
Kwang-sun Kim
Position or Degree
Assistant Professor
Tell Number
+82-51-510-2241(O), 3616(lab)
EMAIL
kwangsun.kim@pusan.ac.kr
HOMEPAGE
http://chemlab.pusan.ac.kr/rnabiochem
Research
Biochemistry

EDUCATION

1994. 3. ~ 1998. 2. Chonbuk National University, B.S. in Chemistry

1998. 3. ~ 2000. 2. Seoul National University, M.S. in Chemistry

2000. 3. ~ 2004. 8. Korea Advanced Institute of Science & Technology (KAIST) , Ph. D. in Chemistry

 

AREAS OF RESEARCH

-Identification and functional analysis of noncoding RNAs

-Modulation of ribonuclease activity by trans-acting regulators for the gene expression control

-Functional identification of RNA aptamers and their uses

-Unraveling mechanistic actions on the erruption of antibiotic resistant bacteria

 

 

EXPERIENCES

2004. 3 ~ 2006. 4. KAIST, Department of Chemistry, Post-doctoral Fellow

2006. 5 ~ 2010. 2. Stanford University, Department of Genetics, Post-doctoral Fellow

(Advisor. Stanley N. Cohen)

2009. 12 ~ 2015. 8. Korea Research Institute of Bioscience and Biotechnology (KRIBB)

, Senior Researcher

2010. 9 ~ 2015. 8. University of Science and Technology (UST), Associate Professor, Adjunct

 

 

SELECTED PUBLICATIONS

1. Kim, T.Y., Bak, G., Lee, J.Y., and Kim, K.S. (2015) “Systematic analysis of the role of bacterial Hfq-interacting sRNAs in the response to antibiotics”. J. Antimicrob. Chemother. doi: 10.1093/jac/dkv042.

 

2. Bak, G., Hong, S.K., Jung, J.Y., and Kim, K.S. (2015) “Construction of a yeast three-hybrid system for identifying Escherichia coli proteins interacting with small noncoding RNAs”. Bull. Kor. Chem. Soc. (In press).

 

3. Kim, K.S., Kim, T., and Pan, J.G. (2015) “In vitro evaluation of ciclopirox as an adjuvant for polymyxin B against gram-negative bacteria”. J. Antibiot. doi:10.1038/ja.2014.164.

 

4. Bak, G., Han, K., Kim, K.S., and Lee. Y. (2015) “Electrophoretic mobility shift assay of RNA-RNA complexes”. Methods Mol. Biol. 1240: 153.

 

5. Kim, T., Lee, J., and Kim, K.S. (2013) “Escherichia coli YmdB regulates biofilm formation independently of its role as an RNase III modulator”. BMC Microbiol. 13: 266. Highly accessed paper.

 

6. Lee, J.Y., Park, H., Bak, G., Kim, K.S., and Lee, Y. (2013) “Regulation of transcription from two ssrS promoters in 6S RNA biogenesis”. Mol. Cells . 36: 227.

 

7. Kim, K.S., Lee, S., and Ryu, C.M. (2013) “Interspecific bacterial sensing through airborne signals modulates locomotion and drug resistance”. Nat. Comm. 4: 1809.

 

8. Kim, K.S., Park, S., Lee, S., Kang, S., Lee, J., Lee, S.G., and Ryu, C.M. (2012) “A novel

fluorescent reporter system for monitoring and identifying RNase III activity and its target RNAs“. RNA Biol.9: 1167.

 

9. Manasherob, R., Miller, C., Kim, K.S., and Cohen, S.N. (2012). “Ribonuclease E modulation of the bacterial SOS response”. PLoS One . 7: e38426.

 

10. Shin, J., Kim, K.S., Ryu, K., Han, K., Lee, Y., and Choi, BS. (2012) “Structural analysis of Escherichia coli C5 protein”. Proteins. 80: 963.

 

11. Han, K., Bak, G., Uhm, K., Kim, K.S., and Lee, Y. (2011) “Construction of an Efficient In Vitro System for Analysis of Transcription from Sigma 54-Dependent pspA Promoter”. Bull. Kor. Chem. Soc. 32: 2129.

 

12. Chae, H., Han, K., Kim, K.S., Park, H., Lee, J.M., and Lee, Y. (2011) “Rho-dependent termination of ssrS (6S RNA) transcription in Escherichia coli : implications for 3 ′ processing of 6S RNA and expression of downstream ygfA . (putative 5-formyl-tetrahydrofolate cyclo-ligase)“. J. Biol. Chem . 286: 114.

 

13. Kook, H., Kim, K.S., Bak, G., Park, H., and Lee, Y. (2010) “Recognition and discrimination of target mRNAs by Sib RNAs, a cis -encoded sRNA”. Nucleic Acids Res. 38: 5851.

 

14. Kim, K.S., Manasherob, M., and Cohen, S.N. (2008) “YmdB: a stress-responsive ribonuclease binding regulator of E. coli RNase III activity”. Genes & Dev . 22: 3497.

 

15. Ko, J., Han, K., Kim, Y., Sim, S., Kim, K.S., Lee, S.J., Cho, B., Lee, K., and Lee, Y. (2008) “Dual function of RNase E for control of M1 RNA biosynthesis in Escherichia coli” Biochemistry . 47: 762.

 

16. Kim, Y., Han, K., Lee, J., Kim, K.S., and Lee, Y. (2007) “Expression of a small protein encoded by the 3' flanking sequence of the Escherichia coli rnpB gene”. Bull. Kor. Chem. Soc. 28: 1010.

 

17. Kim, K.S., Ryoo, H., Lee, J.H., Kim, M., Kim, T., Kim, Y., Han, K., Lee, S., and Lee, Y. (2006) “Selection and Analysis of Genomic Sequence-Derived RNA Motifs Binding to C5 Protein”. Bull. Kor. Chem. Soc . 27: 699.

 

18. Choi, H., Kim, K.S., Park, J.W., Jung, Y.H., and Lee, Y. (2005) “Effect of FIS protein on rnpB transcription inE. coli ”. Mol. Cells. 19: 239.

 

19. Kim, K.S., Sim, S., Ko, J.H., and Lee, Y. (2005) “Alteration of the processing efficiency of M1 RNA at the 3' end affects the cell viability in Escherichia coli “. J. Biol. Chem. 280: 34667.

 

20. Kim, K.S., Sim, S., Ko, J., Cho, B., and Lee, Y. (2004) “Analysis of substrate specificity of the N-terminal catalytic half of RNase E using precursor M1 RNA variants”. J. Biochem . 136: 693.

 

21. Kim, K.S. and Lee, Y. (2004) “Regulation of 6S RNA biogenesis in Escherichia coli : Regulation by switching utilization of both sigma factors and endoribonucleases”. Nucleic Acids Res. 32: 6057.

 

22. Sim, S., Kim, K.S., and Lee, Y. (2002) “3'-end processing of precursor M1 RNA by the N-terminal half of RNase E” FEBS Lett. 529: 225.

 

23. Ko, J., Kim, K.S., Sim, S., Cho, B., Lee, Y. and Park, I. (2000) “The Dps protein shows an ribonuclease activity cleaving the 3' terminal region of the precursor M1 RNA of Escherichia coli ”. J. Kor. Chem. Soc . 44: 87-91.