김 광 선 사진
김 광 선
영문이름
Kim, Kwang-sun
직위(직급)
조교수
전화번호
051-510-2241 (실험실:3616)
이메일
kwangsun.kim@pusan.ac.kr
사이트
http://chemlab.pusan.ac.kr/rnabiochem
연구분야
생화학

연구주제

비코딩 RNA 유전체학 / 리보핵산가수분해 효소 활성 조절인자 / 기능성 RNA 압타머 / 유전자 발현 조절 기작 규명 / 박테리아의 항생제 감응성 조절 신규 기작 및 신규 타겟 - 물질 발굴

 

학력

1994. 3 ~ 1998. 2 전북대학교 화학과 이학사

1998. 3 ~ 2000. 2 서울대학교 화학과 이학석사

2000. 3 ~ 2004. 8 한국과학기술원 화학과 이학박사

 

경력

2004. 9 ~ 2006. 4 한국과학기술원 자연과학대학 연수연구원

2006. 5 ~ 2010. 2 스탠포드 대학교 (Stanford University), 유전학 , 박사후 연구원

2009. 12~ 2015. 8 한국생명공학연구원 선임연구원

2010. 9 ~ 2015. 8 한국과학기술연합대학원 대학교 부교수

 

학술지 게재 논문

 

32. Overexpression of MicA induces production of OmpC-enriched outer membrane vesicles that protect against Salmonella challenge, Choi, H.I., Kim, M., Jeon, J., Han, J.K., Kim, K.S.*, Biochem. Bioph. Res. Comm. 490: 991-996(2017)

 

 

31. Stress-responsively modulated ymdAB-clsC operon plays a role in biofilm formation and apramycin susceptibility in Escherichia coli, Kim, M. and Kim, K.S.*, FEMS Microbiology Letters 364: fnx114(2017)

 

 

30. Ultrasensitive electrochemical detection of miRNA-21 using a zinc finger protein specific to DNA-RNA hybrids, Fang, C.S., Kim, K.S., Yu, B., Jon, S., Kim, M.S., Yang, H., Anal. Chem. 89: 2024.(2017)

 

 

29. YmdB-mediated down-regulation of sucA inhibits biofilm formation and induces apramycin susceptibility in Escherichia coli, Kim, M., Kim, M., Kim, K.S.*, Biochem. Bioph. Res. Comm. 483: 252-257(2017)

 

 

28. Nanovesicles: diagnostic and therapeutic tools in nanoscale medicine, Kim, M., Kim, M., Kim, K.S.*, Applied Science and Convergence Technology 25, 6(2016)

 

 

27. Three rpoS-activating small RNAs in pathways contributing to acid resistance of Escherichia coli, Bak, G., Han, K., Kim, D., Kim, K.S., and Lee, Y., Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria, First Edition. Edited by Frans J. de Bruijn (2016)

 

 

26. Identification of novel sRNAs involved in biofilm formation, motility, and fimbriae formation in Escherichia coli., Bak, G., Lee, J., Suk, S., Kim, D., Lee, J.Y., Kim, K.S., Choi, B.S., and Lee, Y., Sci. Rep. 5: 15287.(2015)

 

 

25. Vaccination with Klebsiella pneumoniae-derived extracellular vesicles protects bacteria-induced lethality via both humoral and cellular immunity, Lee, W.H., Choi, H.I., Hong, S.W., Kim, K.S., Gho, Y.S., and Jeon, S.G. , Exp. Mol. Med. 47: e183.(2015)

 

 

24. Analysis of the roles of small noncoding RNAs in responses to antibiotics and their applications on eradicating MDR bacteria, Kim, T.Y. and Kim, K.S.* , Int. J. Antimicrob. Agents 45: S88(2015)

 


23. Kim, T.Y., Bak, G., Lee, J.Y., and Kim, K.S. (2015) “Systematic analysis of the role of bacterial Hfq-interacting sRNAs in the response to antibiotics”. J. Antimicrob. Chemother. doi: 10.1093/jac/dkv042 (IF=5.439).

 

22. Bak, G., Hong, S.K., Jung, J.Y., and Kim, K.S. (2015) “Construction of a yeast three-hybrid system for identifying Escherichia coli proteins interacting with small noncoding RNAs”. Bull. Kor. Chem. Soc. (In press) (IF=0.835).

 

21. Kim, K.S., Kim, T., and Pan, J.G. (2015) “In vitro evaluation of ciclopirox as an adjuvant for polymyxin B against gram-negative bacteria”. J. Antibiot. doi:10.1038/ja.2014.164 (IF=2.041).

 

20. Bak, G., Han, K., Kim, K.S., and Lee. Y. (2015) “Electrophoretic mobility shift assay of RNA-RNA complexes”. Methods Mol. Biol. 1240: 153.

 

19. Kim, T., Lee, J., and Kim, K.S. (2013) “Escherichia coli YmdB regulates biofilm formation independently of its role as an RNase III modulator”. BMC Microbiol. 13: 266. Highly accessed paper (IF=2.98).

 

18. Lee, J.Y., Park, H., Bak, G., Kim, K.S., and Lee, Y. (2013) “Regulation of transcription from two ssrS promoters in 6S RNA biogenesis”. Mol. Cells . 36: 227 (IF=2.242).

 

17. Kim, K.S., Lee, S., and Ryu, C.M. (2013) “Interspecific bacterial sensing through airborne signals modulates locomotion and drug resistance”. Nat. Comm. 4: 1809 (IF=10.742).

 

16. Kim, K.S., Park, S., Lee, S., Kang, S., Lee, J., Lee, S.G., and Ryu, C.M. (2012) “A novel

fluorescent reporter system for monitoring and identifying RNase III activity and its target RNAs“. RNA Biol. 9: 1167 (IF=5.377).

 

15. Manasherob, R., Miller, C., Kim, K.S., and Cohen, S.N. (2012). “Ribonuclease E modulation of the bacterial SOS response”. PLoS One . 7: e38426 (IF=3.534).

 

14. Shin, J., Kim, K.S., Ryu, K., Han, K., Lee, Y., and Choi, BS. (2012) “Structural analysis of Escherichia coli C5 protein”. Proteins. 80: 963 (IF=2.921).

 

13. Han, K., Bak, G., Uhm, K., Kim, K.S., and Lee, Y. (2011) “Construction of an Efficient In Vitro System for Analysis of Transcription from Sigma 54-Dependent pspA Promoter”. Bull. Kor. Chem. Soc. 32: 2129 (IF=0.835).

 

12. Chae, H., Han, K., Kim, K.S., Park, H., Lee, J.M., and Lee, Y. (2011) “Rho-dependent termination of ssrS (6S RNA) transcription in Escherichia coli : implications for 3 processing of 6S RNA and expression of downstream ygfA . (putative 5-formyl-tetrahydrofolate cyclo-ligase)“. J. Biol. Chem . 286: 114 (IF=4.6).

 

11. Kook, H., Kim, K.S., Bak, G., Park, H., and Lee, Y. (2010) “Recognition and discrimination of target mRNAs by Sib RNAs, a cis -encoded sRNA”. Nucleic Acids Res. 38: 5851 (IF=8.808).

 

10. Kim, K.S., Manasherob, M., and Cohen, S.N. (2008) “YmdB: a stress-responsive ribonuclease binding regulator of E. coli RNase III activity”. Genes & Dev . 22: 3497 (IF=12.639).

 

9. Ko, J., Han, K., Kim, Y., Sim, S., Kim, K.S., Lee, S.J., Cho, B., Lee, K., and Lee, Y. (2008) “Dual function of RNase E for control of M1 RNA biosynthesis in Escherichia coli” . Biochemistry . 47: 762 (IF=3.194).

 

8. Kim, Y., Han, K., Lee, J., Kim, K.S., and Lee, Y. (2007) “Expression of a small protein encoded by the 3' flanking sequence of the Escherichia coli rnpB gene”. Bull. Kor. Chem. Soc. 28: 1010.

 

7. Kim, K.S., Ryoo, H., Lee, J.H., Kim, M., Kim, T., Kim, Y., Han, K., Lee, S., and Lee, Y. (2006) “Selection and Analysis of Genomic Sequence-Derived RNA Motifs Binding to C5 Protein”. Bull. Kor. Chem. Soc . 27: 699 (IF=0.835).

 

6. Choi, H., Kim, K.S., Park, J.W., Jung, Y.H., and Lee, Y. (2005) “Effect of FIS protein on rnpB transcription in E. coli ”. Mol. Cells. 19: 239 (IF=2.242).

 

5. Kim, K.S., Sim, S., Ko, J.H., and Lee, Y. (2005) “Alteration of the processing efficiency of M1 RNA at the 3' end affects the cell viability in Escherichia coli “. J. Biol. Chem. 280: 34667 (IF=4.6).

 

4. Kim, K.S., Sim, S., Ko, J., Cho, B., and Lee, Y. (2004) “Analysis of substrate specificity of the N-terminal catalytic half of RNase E using precursor M1 RNA variants”. J. Biochem . 136: 693 (IF=3.073).

 

3. Kim, K.S. and Lee, Y. (2004) “Regulation of 6S RNA biogenesis in Escherichia coli : Regulation by switching utilization of both sigma factors and endoribonucleases”. Nucleic Acids Res. 32: 6057 (IF=8.808).

 

2. Sim, S., Kim, K.S., and Lee, Y. (2002) “3'-end processing of precursor M1 RNA by the N-terminal half of RNase E” FEBS Lett. 529: 225 (IF=3.986).

 

1. Ko, J., Kim, K.S., Sim, S., Cho, B., Lee, Y. and Park, I. (2000) “The Dps protein shows an ribonuclease activity cleaving the 3' terminal region of the precursor M1 RNA of Escherichia coli ”. J. Kor. Chem. Soc . 44: 87.